The second season of T.U.F.F. Puppy was released on DVD in January 8, 2015. It was released as an Amazon exclusive and is presented in a DVD-R Format.
Disc 1
- Pup Daddy / Candy Cane-ine
- Freaky Spy-Day / Dog Tired
- Dog House / Time Waits for No Mutt
- Love Bird / Bluff Puppy
- Bark to the Future / Lights, Camera, Quacktion
- Close Encounters of the Doomed Kind / Golden Retriever
- Rat Trap / Agent of the Year
- T.U.F.F. Choices / Sob Story
- Hush Puppy / Quacky Birthday
Disc 2
- Happy Howl-O-Ween
- 'Til Doom Do Us Part
- Barking Tall / Bad Eggs
- Bark to Nature / Mutts and Bolts
- Carbon Copies / T.U.F.F. Cookies
- Subliminal Criminal / Acting T.U.F.F.
- T.U.F.F. Sell / Tattle Tale
- A Tale of Two Kitties / Pup in the Air
- True Spies / Bagel and the Beast