The first season of T.U.F.F. Puppy was released on DVD on June 23, 2014. It was released as an Amazon exclusive and is presented in a DVD-R format.
- Purr-fect Partners / Doom-mates
- Mall Rat / Operation: Happy Birthday
- Snapnapped / Mom-A-Geddon
- Cruisin' For A Bruisin' / Puppy Love
- Toast Of T.U.F.F. / Share-A-Lair
- Dog Daze / Internal Affairs
- Chilly Dog / The Doomies
- Thunder Dog / Snap Dad
- Iron Mutt / The Wrong Stuff
- Watch Dog / Dog Dish
- Forget Me Mutt / Mind Trap
- Kid Stuff / Super Duper Crime Busters!
- Mission: Really Big Mission
- Frisky Business / Hot Dog
- Disobedience School / The Dog Who Cried Fish
- Doom and Gloom / Law and Odor
- The Rat Pack / Booby Trap
- The Curse of King Mutt / Bored of Education
- Snappy Campers / Lucky Duck
- Guard Dog / Dog Save the Queen
- A Doomed Christmas
- Big Dog on Campus / Dog's Best Friend
- Dudley Do-Wrong / Puppy Unplugged
- Top Dog / Quack in The Box
- Monkey Business / Diary of a Mad Cat
- Lie Like a Dog / Cold Fish
- The last 6 episodes of season 1 were originally produced in a widescreen format. However, for unknown reasons, the episodes have been cropped to a full screen format on this DVD set.
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